Layered-IHC (L-IHC): A novel and robust approach to multiplexed immunohistochemistry

Page 1 © 2010 20/20 GeneSystems, Inc. The need for multiplex detection of tissue biomarkers. There is a constant and growing demand for increased biomarker analysis in human tissue specimens. Analysis of tissue biomarkers is key to understanding the activation status of particular pathways in specific cell types and can be indicative of disease diagnosis, prognosis, prediction of therapeutic efficacy and monitoring of treatment outcomes. This information is of further importance in identifying and validating novel drug targets. While in certain instances detection of single biomarkers is sufficient, it is rapidly becoming clear that both panels of multiple markers and variations in biomarker levels relative to each other is more informative and more diagnostic of cell and disease status.